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Registros recuperados: 33 | |
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Borelli,Beatriz M.; Ferreira,Elaine G.; Lacerda,Inayara C.A.; Santos,Deise A.; Carmo,Luiz S.; Dias,Ricardo S.; Silva,Maria Crisolita C.; Rosa,Carlos A.. |
Canastra cheese is produced from raw cow's milk, and it is made at the farmhouse level using artisanal procedures and natural starters. The aim of this work was to determine the main hygienic-sanitary indicators and enterotoxigenic staphylococcal strains present during the manufacturing of traditional cheese of Serra da Canastra region, Minas Gerais state, Brazil. Samples from 10 farms were studied, and they included: water employed in the process, raw milk, natural starters, cheese curd before salting and cheese after five days of ripening. All water samples exhibited faecal coliform contamination above the maximum acceptable value recommended by Brazilian standards. Pseudomonas aeruginosa and sulfite-reducing clostridia were also isolated from the water... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artisanal cheese; Coliforms; Staphylococcus; Enterotoxin. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400026 |
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Borelli,B.M.; Lacerda,I.C.A.; Brandão,L.R.; Vianna,C.R.; Ferreira,M.C.; Gomes,F.C.O.; Carmo,L.S.; Heneine,L.G.D.; Rosa,C.A.. |
The population dynamics of Staphylococcus spp. was studied during the ripening of Canastra Minas cheese at three farms located in the State of Minas Gerais, Brazil. The presence of coagulase (coa), thermonuclease (nuc), and enterotoxin (sea, seb, sec, and sed) genes was investigated in Staphylococcus strains isolated during the 60-day cheese-ripening period. The presence of the staphylococcal enterotoxins A, C, and D was also investigated in the cheese samples. Cheese samples that were matured for 0, 7, 15, 30, and 45 days presented staphylococci counts from 10³ to 10(8)cfu/g. All isolates considered coagulase-positive by physiological tests had the coa gene. However, no association was observed between the results obtained with biochemical tests and those... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canastra Minas cheese; Staphylococcus; Enterotoxin. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000200028 |
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Costa,Dayane de Melo; Kipnis,André; Leão-Vasconcelos,Lara Stefânia Netto de Oliveira; Rocha-Vilefort,Larissa Oliveira; Telles,Sheila Araújo; André,Maria Cláudia Dantas Porfírio Borges; Tipple,Anaclara Ferreira Veiga; Lima,Ana Beatriz Mori; Ribeiro,Nádia Ferreira Gonçalves; Pereira,Mayara Regina; Prado-Palos,Marinésia Aparecida. |
The aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and saliva sampling were performed with 149 health care workers. Antimicrobial resistance was determined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were performed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were determined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Health personnel; Occupational health; Staphylococcus; Methicillin resistance; Oncology service; Hospital. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300006 |
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Coutinho,Vivian de Lima Spode; Paiva,Rodrigo Minuto; Reiter,Keli Cristine; de-Paris,Fernanda; Barth,Afonso Luis; Machado,Alice Beatriz Mombach Pinheiro. |
INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus; Resistance; Erm genes; Macrolides. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004 |
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Cunha,Rodrigo Casquero; Rosa,Michelle Dias Hornes da; Silva,Cleomar da; Santos,Francisco Denis Souza; Leite,Fábio Pereira Leivas. |
ABSTRACT: The genus Staphylococcus comprises some of the most important pathogenic bacteria for both humans and animals. It is responsible for bovine mastitis and canine otitis, besides being present in the microbiota of animals and as a contaminant in food. Its pathogenesis is related to the formation of capsule and biofilm, which contribute to its infectivity. The objective of this study was to observe the production of slime layer and formation of biofilm, which are related to the resistance to antimicrobial agents and presence of icaA and icaD genes, in 41 isolates of Staphylococcus spp. from different origins, provided by the Universidade Federal de Pelotas (UFPEL), Laboratório Regional de Diagnóstico (LRD). Strains of Staphylococcus spp. were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus; Biofilm; Slime layer. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500453 |
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Delarras, C; Guichaoua, C.; Caprais, Marie-paule. |
129 strains of Micrococcaceae were isolated from food of animal origin (minced meat, cakes with confectioner's custard) on Baird-Parker medium. 120 were Staphylococcus and 9 Micrococcus, according to the ID 32 STAPH-System (1989). The results were analyzed using a computer program. 60% of these 120 Staphylococcus strains of animal origin were coagulase positive S. aureus (aurease+, Rapidec Staph technique). 46% were resistant to novobiocin (5 microg/ml), 13 to 44% were identified with negative discordant tests using this micromethod. 44% produced no acetoin, 35% had no urease and 15% no arginine dihydrolase. 48 Staphylococcus strains (40%) were identified as human coagulase negative Staphylococcus species (strains: 39; species: 9) or as animal species... |
Tipo: Text |
Palavras-chave: Staphylococcus; Nitrofurantoin; Aurease; ID 32 STAPH; Numerical identification. |
Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00071/18273/15838.pdf |
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Denamiel,G; Puigdevall,T; Más,J; Albarellos,G; Gentilini,E. |
El objetivo de este trabajo fue conocer la prevalencia de especies y perfil de resistencia a betalactámicos en estafilococos aislados de infecciones en caninos y felinos. En perros, de 207 aislamientos, el porcentaje de las especies identificadas fue: S. intermedius 29,5%, S. aureus y S. simulans 20,8%, S. saprophyticus 12,6%, S. chromogenes 10,6%, S. haemolyticus 3,9% y S. epidermidis 1,9%. De 63 aislamientos en gatos: S. simulans 33,3%, S. intermedius y S. aureus 15,4%, S. saprophyticus 11,1%, S. haemolyticus 6,34%, S. chromogenes y S. epidermidis 3,5%. En perros, el porcentaje de resistencia a penicilina fue: S. intermedius 47,5%, S. aureus 34,8% y S. saprophyticus 7,7%. Los aislamientos que expresaron meticilina resistencia correspondieron: S.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Betalactámico; Resistencia; Staphylococcus; Perro; Gato. |
Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982009000200006 |
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Dias,R.S.; Carmo,L.S.; Heneine,L.G.D.; Rocha,P.H.; Barbosa,C.F.; Rodrigues,R.J.; Linardi,V.R.. |
Acute toxicity test (LD-50) using toxic shock syndrome toxin (TSST-1) was tested in BALB/c, C57BL/6 and Swiss mice. Animals (n = 10) were intraperitoneally injected with TSST-1 (0.01-10.0µg/mouse) followed 4h later by potentiating dose of lipopolysaccharide (75.0µg of LPS - E. coli O111:B4) and cumulative mortality was recorded over 72h. Control animals received either TSST-1 or LPS alone. The data were submitted to qui-Square test and acute toxicity test was calculated by probit analysis (confidence limits expressed as µg toxin/kg). BALB/c mice was the most sensitive (20.0µg/kg, 95% confidence limits: 9.0-92.0) followed by C57BL/6 (38.5µg/kg, 95% confidence limits: 9.11- 401.6). Data from Swiss mice was not conclusive, indicating only low sensitivity.... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Mice; TSST-1; Acute toxicity (LD-50); Staphylococcus. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100024 |
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Lamaita,H.C.; Cerqueira,M.M.O.P.; Carmo,L.S.; Santos,D.A.; Penna,C.F.A.M.; Souza,M.R.. |
Analisaram-se 80 amostras de leite cru refrigerado a 4°C e estocado por 48 horas em tanques refrigeradores de propriedades rurais do estado de Minas Gerais quanto à contagem e identificação de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas (SE) e da toxina da síndrome do choque tóxico (TSST-1). Staphylococcus sp. foi detectado em 100% das amostras de leite de tanque refrigerador em contagens que variaram de 1,0 × 10(5) a 2,5 × 10(7) UFC/ml (média = 5,60 log UFC/ml; s = 0,53 e CV = 9,5%). Isolaram-se e identificaram-se 436 estirpes como: S. aureus, S hyicus, S. epidermidis, S. intermedius, S. cohnii, S. sciuri, S. schleirferi e S. delphini. As estirpes de mesmo perfil bioquímico, oriundas da mesma amostra, foram agrupadas (pools) e induzidas... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leite cru; Staphylococcus; Enterotoxina; TSST-1. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500017 |
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Loguercio,Andrea Pinto; Groff,Ana Cláudia Mello; Pedrozzo,Andréia Folleto; Witt,Niura Mazzini; Silva,Mariana Sá e; Vargas,Agueda Castagna de. |
Este trabalho foi desenvolvido com o objetivo de avaliar a atividade in vitro do extrato alcoólico de própolis, contra agentes da mastite bovina, comparando-o aos principais antimicrobianos utilizados no tratamento convencional. Foram utilizados 36 isolados coagulase-positivos de Staphylococcus sp. e 27 isolados de Streptococcus sp.; 94,4% dos Staphylococcus sp. e 85,2% dos Streptococcus sp. foram susceptíveis ao extrato de própolis. |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Staphylococcus; Streptococcus; Susceptibilidade; Antimicrobianos. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000200021 |
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Machado-Ferreira,Erik; Vizzoni,Vinicius Figueiredo; Piesman,Joseph; Gazeta,Gilberto Salles; Soares,Carlos Augusto Gomes. |
Abstract Ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. In the Americas, the Ixodidae ticks Amblyomma cajennense are responsible for severe impact on livestock and public health. In the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with A. cajennense eggs from females sampled in distinct geographical sites in southeastern Brazil. Additional comparative analysis of the culturable bacteria from Anocentor nitens, Rhipicephalus sanguineus and Ixodes scapularis tick eggs were also performed. 16S rRNA gene sequence analyses identified 17 different bacterial types identified as Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas fluorescens,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amblyomma cajennense; Tick eggs bacteria; Staphylococcus; Ixodidae. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400477 |
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Registros recuperados: 33 | |
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